From ABI Sequence Data to LASERGENE. We use Taq dye terminator chemistry rather than dye primer sequencing because we are currently interested in sequencing PCR products of viral genomes amplified from clinical specimens (1,2). We prefer to sequence PCR products directly rather than after cloning them to avoid seeing unrepresentative sequences. To be able to look at the distribution of sequences in a population (the quasispecies), we dilute the starting specimen until amplification is achieved from a single molecule (3). Alternatively, if the sequence population is relatively homogeneous, sequencing from a bulk PCR amplification will be accurate since Taq misincorporation errors will be effectively diluted out. MegAlign; PrimerSelect; Protean. In addition DNASTAR have also supplied copies of the instruction manual and tutorial exercises. PROTEAN - analyses protein sequences for predicted properties; SEQMAN. SeqMan, Free Download by DNASTAR, Inc. MEGA is a integrated tool for conducting automatic and manual sequence alignment. DNASTAR - Training and Support for Sequence Analysis Software, Next- Gen Sequencing Software, Primer Design Software, Gene Expression Software'What’s not to like? The people at DNASTAR are responsive.'Douglas Rhoads. The Complete Genome Sequence of Escherichia coli DH10B. Pseudogenes were identi
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December 2016
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